— no clinical, pathological or serological evidence of Aujeszky's disease has been recorded in the previous 12 months in the holding in question,

— no clinical, pathological or serological evidence of Aujeszky's disease has been recorded in the previous 12 months in the holdings located in an area of 5 km surrounding the holding of origin of the pigs; however, this provision shall not apply if, in these latter holdings, disease monitoring and eradication measures have been regularly applied under the supervision of the competent authority and in accordance with the eradication plan referred to in point (b), and these measures have effectively prevented any spread of disease to the holding in question,

— vaccination against Aujeszky's disease has not been carried out for at least 12 months,

— the pigs have been subjected on at least two occasions at a distance of at least four months to a serological survey for the presence of ADV-gE or ADV-gB or ADV-gD antibody or to the whole Aujeszky's disease virus. This survey must have shown the absence of Aujeszky's disease and that vaccinated pigs have been free from gE antibodies,

— no pigs have been introduced from holdings of a lower animal health status as regards Aujeszky's disease in the previous 12 months, unless they have been tested for Aujeszky's disease with negative results;

— have not been vaccinated,

— have been kept isolated in accommodation approved by the competent authority, during the 30 days prior to movement, and in such a way that any risk of spreading Aujeszky's disease to these pigs is prevented,

— must have lived in the holding of origin or in a holding of an equivalent status since birth, and have remained in the holding of origin for at least:

— have been subjected with negative results to at least two serological tests for ADV-gB or ADV-gD or the whole Aujeszky's disease virus, at a distance of at least 30 days between each test. However, in case of pigs less than four months old, the serological test for ADV-gE may also be used. Sampling for the last test must be performed within 15 days prior to shipment. The number of pigs tested in the isolation unit must be sufficient to detect:

— However, the first of the two tests shall not be necessary if:

— they come from a holding which fulfils the conditions laid down in Article 1(c), or

— they have been vaccinated against Aujeszky's disease at least 15 days prior to their shipment and come from a holding of origin where:

— they have not been vaccinated and they proceed from a holding where:

— Member States or regions listed in Annex II, and

— a holding which fulfils the requirements of Article 1(c); or

— Aujeszky's disease must be compulsorily notifiable in the Member State of origin,

— a plan for the control and eradication of Aujeszky's disease is in place in the Member States or region of origin, which fulfils the criteria laid down in Article 1(b),

— no clinical, pathological or serological evidence of Aujeszky's disease has been recorded in the previous 12 months in the holding of origin of the pigs in question,

— the pigs must have been isolated in accommodation approved by the competent authority for the 30 days immediately prior to movement and kept isolated in such a way that any risk of spreading of Aujeszky's disease is prevented,

— the pigs must have been subjected, with negative results, to a serological test for the presence of gE antibodies. Sampling for the last test must be performed within 15 days prior to shipment. The number of pigs tested must be sufficient to detect 2 % seroprevalence with 95 % confidence in these pigs,

— the pigs must lave lived in the bolding of origin or in a holding of an equivalent status since birth, and have remained in the holding of origin for at least 90 days.

— Member States or regions listed in Annex II, and

— a holding which fulfils the requirements of Article 1(c); or

— Aujeszky's disease must be compulsorily notifiable in the Member State of origin,

— a plan for the control and eradication of Aujeszky's disease is in place in the Member States or region of origin, which fulfils the criteria laid down in Article 1(b),

— no clinical, pathological or serological evidence of Aujeszky's disease has been recorded in the previous 12 months in the holding of origin of the pigs in question,

— a serological survey for Aujeszky's disease, demonstrating its absence and that vaccinated pigs have been free from gE antibodies, has been carried out in the holding of origin and between 45 and 170 days prior to shipment,

— the pigs must either have lived in the holding of origin since birth or have remained in such holdings for at least 30 days after introduction from a holding of an equivalent status, where a serological survey equivalent to the one referred to in the fourth indent above has been carried out.

(a) The sensitivity of the test must be of such a level that the following Community reference sera are scored positive:

(b) The specificity of the test must be of such a level that the following Community reference sera are scored negative:

(c) For batch control, Community reference serum ADV 1 must be scored positive at 1:8 dilution and one of the Community reference serum sera from ADV-gE G to ADV-gE Q, as listed in point (b), must be scored negative.

For batch control of ADV-gB and ADV-gD kits, Community reference serum ADV 1 must be scored positive at the dilution of 1:2 and Community reference serum Q referred to in (b) should be scored negative.

(d) The institutes listed below will, in addition, be responsible for checking the quality of the ELISA method in each Member State, and in particular for producing and standardising national reference sera according to the Community reference sera.