Characterization of Liposome-based Nanomaterials
Описание
In order to provide guidelines to design and perform a robust and reliable physical-chemical characterization of liposome-based nanomaterials, and to support method development with a specific focus on their inflammation-inducing potential the following data have been produced:
- Physical-chemical characterization
- Interference test
- Liposome Cytotoxicity
- Effects of Liposomes on Complement Activation
- Inflammatory response
Out of eight differently functionalized liposomes selected as "case-studies", three passed the physical-chemical characterization ( in terms of size-distribution, homogeneity and stability) and the screening for bacterial contamination (sterility and apyrogenicity). Although all three were non-cytotoxic when tested in vitro, they showed a different capacity to activate human blood cells. HSPC/CHOL-coated liposomes elicited the production of several inflammation-related cytokines, while DPPC/CHOL- or DSPC/CHOL-functionalized liposomes did not.
EuroVoc области
Ресурси
- Complement activation Excel XLSX
- Hydrodynamic diameter measured by applying the Flow-field-flow fractionation (AF4) system Excel XLSX
- Inflammatory response (Origin project) ZIP
- Liposome Cytotoxicity (GraphPad Prism) ZIP
- Liposome interference with the chromogenic LAL assay’ readout of pNA at 405 nm Excel XLSX
- Liposome interference with the chromogenic LAL assay’ readout of pNA+ azo-dye at 540 nm Excel XLSX
- Particle size distribution (PSD) and polydispersity index (PDI) of liposomes performed by Dynamic Light Scattering (DLS) Excel XLSX
- Stokes radius of four selected liposomes (F10103, F10102, F20103A and F20104A) measured by analytical ultracentrifuge (AUC) Excel XLSX
- UV-Vis spectra of liposomes Excel XLSX
- Идентификатор
- http://data.europa.eu/89h/1a57e3d3-f5a3-4ca9-9e67-ec85569e6562
- Целева страница
- http://data.europa.eu/89h/1a57e3d3-f5a3-4ca9-9e67-ec85569e6562
- Дата на публикуване
- 2020-12-01
- Дата на промяна
- 2020-12-01
- Периодичност на добавяне
- непознат
- Catalogue
- European Union Open Data Portal